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KMID : 0855520120250030131
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Korean Journal of Physical Anthropology
2012 Volume.25 No. 3 p.131 ~ p.136
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Evaluation of KRAS let-7 lcs6 Polymorphism in Korean Populations by Pyrosequencing
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Lee Jae-Ho
Kim Dae-Kwang
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Abstract
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A single nucleotide polymorphism (SNP) in the 3′-untranslated region of the KRAS oncogene (rs61764370) was recently reported to act as a genetic marker for increased risk of developing human cancers. The frequency of the variant allele is 5.8% in world population, however, there is no study in Korean population with large cases. In present study, we evaluated the KRAS let-7 lcs6 SNP in Korean population for the first time.
Genotyping was performed in 178 randomized subjects. DNA was extracted and SNP was evaluated by using PCR and pyrosequencing to identify the T or G allele of the KRAS let-7 lcs6 SNP.
As a result, only T allele was found in all subjects and quantitative sequencing result showed that the proportion of G allele was extremely low.
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KEYWORD
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KRAS, Single nucleotide polymorphism (SNP), let-7 lcs6
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